Product Datasheet

Product Name

Product number

SsL-90-01

Product type 

Enzyme, glycyl-glycine-endopeptidase

Source

Staphylococcus simulans biovar staphylolyticus

Description

Chromatographically purified, high purity and specific activity from a recombinant S. simulans biovar staphylolyticus strain. Protein size: 26 kDa

Solubility

Soluble in water or aqueous buffer (pH 5-8) (5 mg/ml)

Natural antibacterial enzyme, which belongs to the class of proteases and is active against staphylococci (e.g. pathogenic S. aureus)

Mode of action:

• cleavage of the pentaglycine interpeptide cross bridge, which is characteristic for the genus Staphylococcus

Intended applications:

• ·Treatment of multidrug resistant strains of Staphylococcus aureus

• Lysis of staphylococci for further molecular biological processing such as plasmid isolation

• Generation of protoplasts from staphylococci as prerequisite for  transformation

• Hydrolysis of staphylococcal murein

Storage buffer 

-

Form

white powder (lyophilized), contains no buffer salts or additives!

Storage instructions

Lyophilisate: store dry at -20°C

Solution: store at -20°C, at 4°C stable for some days.

Purification notes 

Chromatographically purified. Purity ~90% (by SDS-PAGE)

Specific activity

Specific activity is ≥ 1800 U/mg protein

Unit definition: 1 U is defined as the activity that reduces the turbidty (A₆₀₀) of a suspension of S. aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 and 25°C in a 1 ml reaction volume (corresponds to a DOD₆₀₀ of -0,0125/min in a 1 ml cuvette)

Safety Notes 

For research use only! Not intended for therapeutic use! Do not breathe dust!

 References

Schindler, C.A. and V.T. Schuhardt. (1964) Lysostaphin: a new bacteriolytic agent for the stapylococci. Proc. Nat. Acad. Sci. U.S.A. 51: 414-421.

Recsei, P.A., Gruss, A.D., and Novick, R.P. (1987) Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans. Proc. Nat. Acad. Sci., U.S.A. 84: 1127-1131.

Heinrich, P., Rosenstein, R., Böhmer, M., Sonner, P., Götz, F. (1987). The molecular organi-zation of the lysostaphin gene and its sequences repeated in tandem. Mol Gen Genet. 209: 563-569.